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Thul Peter J Åkesson Lovisa Wiking Mikaela Mahdessian Diana Geladaki Aikaterini Ait Blal Hammou. We present a subcellular map of the human proteome called the Cell Atlas to facilitate functional exploration of individual proteins and their role in human biology and disease.
Синяя социальная система управления закладками и публикациями.
A subcellular map of the human proteome. Subcellular map can be used to refine existing protein-protein interaction networks and provides an important resource to deconvolute the highly complex architecture of the human. We present a subcellular map of the human proteome called the Cell Atlas to faci-litate functional exploration of individual pro-teinsandtheirroleinhumanbiologyanddisease. Resolving the spatial distribution of the human proteome at a subcellular level can greatly increase our understanding of human biology and disease.
Here we present a comprehensive image-based map of subcellular protein distribution the Cell Atlas built by integrating transcriptomics and antibody-based immunofluorescence microscopy with validation by mass spectrometry. Paper I of this thesis presents a subcellular map of the human proteome where the spatial distribution of 12003 human proteins was mapped into 30 subcellular structures half of which were not previously localized. Besides providing a valuable dataset for cell biology this study is the first to reveal the spatial complexity of human cells with proteins localizing to multiple compartments and.
Here we present an image-based map of the subcellular distribution of 12051 human proteins in 22 cell lines based on an integrative approach using transcriptomics and antibody-based. A subcellular map of the human proteome. We present a subcellular map of the human proteome called the Cell Atlas to facilitate functional exploration of individual proteins and their role in human biology and disease.
RATIONALE Immunofluorescence IF microscopy was used to systematically resolve the spatial distribution of human proteins in cultivated cell lines and map them to cellular compartments and substructures with single. The Cell Atlas team has been working on the generation of the map of the subcellular human proteome for over 10 years. The project has shifted character over the years from automation and establishing robust assay platforms to generating massive amounts of data by performing thousands of immunostainings per week to the analysis and interpretation of the results.
These genes encode the cells proteome. The full set of proteins that perform functions in the cell. In paper I we show that RNA sequencing is a necessary tool for studying the proteome of the human cell.
By studying the proteome and proteins localization in the cell information can be assembled on how the cell functions. Image-based methods allow for detailed spatial resolution of. We present a subcellular map of the human proteome called the Cell Atlas to facilitate functional exploration of individual proteins and their role in human biology and diseaseRATIONALEImmunofluorescence IF microscopy was used to systematically resolve the spatial distribution of human proteins in cultivated cell lines and map them to cellular compartments and.
Resolving the spatial distribution of the human proteome at a subcellular level can greatly increase our understanding of human biology and disease. Here we present a comprehensive image-based map of subcellular protein distribution the Cell Atlas built by integrating transcriptomics and antibody-based immunofluorescence microscopy with validation by mass spectrometry. Mapping the in situ.
We have curated an in-depth subcellular proteomic map of primary human CD4 T cells divided into cytosolic nuclear and membrane fractions generated by an optimized fractionation and HiRIEF-LC-MSMS workflow for limited amounts of primary cells. The subcellular proteome of T cells was mapped under steady state conditions as well as upon 15 min and 1 h of T cell receptor TCR. Синяя социальная система управления закладками и публикациями.
Log in with your OpenID-Provider. To develop a draft map of the human proteome by systematically identifying and annotating protein-coding genes in the human genome we carried out. A subcellular map of the human proteome.
Thul Peter J Åkesson Lovisa Wiking Mikaela Mahdessian Diana Geladaki Aikaterini Ait Blal Hammou. Show all 43 Publisher. Science American Association for the Advancement of Science 2017-05-26 Vol356 6340 peaal3321.
Previously we used APEX to map the proteome of the human mitochondrial matrix Rheeetal2013In thepresent studywe focused on the proteome of the mitochondrial intermembrane space IMS which lies between the inner mitochondrial mem-brane IMM and outer mitochondrial membrane OMM Fig-ure 1A. The IMS cannot be purified by traditional approaches such as density centrifugation. A total of 12003 proteins targeted by 13993 antibodies were classified into one or several of 30 cellular compartments and substructures altogether defining the proteome of 13 major organelles.