Centrifuge k factor calculator

Most researchers refer to the maximum speed of a rotor to compare efficiency. By using the k-factor a measure of the sedimentation distance.

Calculate the volumetric ow rate Q V t D2 p ˆ p ˆ f2 18 f lnr 2r 1 ˇ r2 2 r 2 1 h m3s 1 I What happens if we operate a ow rate.

Centrifuge k factor calculator. The information presented by the Intellifuge calculator should be used for estimation purposes and should be used as a reference only. For exact information refer to the specific rotor instruction for use IFU. Additionallyk-factor is calculated and therefore values.

Most researchers refer to the maximum speed of a rotor to compare efficiency. This can be misleading as factors such as tube geometry and temperature contribute to rotor performance and must also be considered. Separation is affected by maximum speed and radius which together determine maximum g-force.

To calculate the Unknown quantities enter the known value and select its Compute from button. When entering RPM or RCF values do not use a comma. Go to Run Time Conversion Using k-Factors.

Go to Whats New in Centrifugation. Go to Centrifuge Home Page. By using the k-factor a measure of the sedimentation distance.

This will tell you how long it will take the particles to settle at the bottom of the test tube. The k-factor represents the pelleting efficiency of a centrifugation system at maximum rotational speed. Centrifuge Rotor Speed Calculator.

Choose the make and model of your centrifuge rotor and the values will be entered automatically. Or for a rotor not on the list choose a Custom Rotor by clicking the Enter parameters manually. K-factor however is still an excellent determination for rotor efficiency as it includes all parameters to replicate a centrifugation step and clearly defines the proficiency of a centrifuge-rotor system.

The Determinants of k-Factor The most important contributors to rotor efficiency are the maximum speed maximum radius r. In the calculations of centrifuge power one should distinguish between starting power N start and operation period power N op. The starting power is a sum of.

Swinging-bucket centrifuge rotors feature a full pathlength slow run time and excellent resolution. The swinging bucket rotor allows the tube to change angle during the run. IKA introduces a brand new micro centrifuge ideal for applications which do not require ultra high speeds eg micro-filtrations and cell separations.

These centrifuges feature a compact design and a small footprint which requires minimal bench space. A transparent cover allows for constant observation of the centrifuge and it can be used with both microcentrifuge and PCR strip tubes. Use the RCF or RPM calculator below to determine the RCF or RPM value for your centrifugation cycle.

If you need help determining the radius of your centrifuge configuration simply fill out the form below or give us a call at 1-866-370-4388. Where k 1 is the k -factor of the SW 32 Ti rotor and k 2 is the k -factor of the SW 28 rotor. T 2 is the run duration of a previous method and t 1 refers to the unknown spin time for the SW 32 Ti.

Where g is the relative centrifugal force R is the radius of the rotor in centimeters and S is the speed of the centrifuge in revolutions per minute. Values of RCF in units of times gravity g for common microcentrifuge rotor radii appear in the following conversion table. Especially for ultracentrifugation the k-factor is still relevant.

How to select the right centrifuge for your application If you follow a given protocol make sure to use the same type of rotor and apply the given relative centrifugal force rcf as well as the same temperature and running time. A low K factor indicates a higher pelleting efficiency. Therefore the K factor can be a useful metric for comparing the speed at which particles will pellet across a range of rotors.

Here tubes are held in a fixed position at angles from 250 to 400 to the vertical axis of rotation. To convert between centrifugation speed RPM Revolution Per Minute and centrifugation force RCF Relative Centrifugal Force. Choose a rotor type or input custom rotor parameters.

Type in RPM or RCF. The other variable will be computed automatically as you type. K-factor is always automatically calculated.

Calculating the centrifuges throughput Q Once we know how long a particle should be in the centrifuge we can calculate a feed owrate Q. The volume of uid in the centrifuge is V ˇ r2 2 r2 1 h. Calculate the volumetric ow rate Q V t D2 p ˆ p ˆ f2 18 f lnr 2r 1 ˇ r2 2 r 2 1 h m3s 1 I What happens if we operate a ow rate.

Y factor and K factor represent part constants used in formulas to calculate the developed length of flat sheet metal required to make a bend of a specific radius and angle in a design. Y factor and K factor are defined by the location of the sheet metal materials neutral bend line with respect to the thickness. Fixed Angle Centrifuge Rotors.

Fixed-angle rotors feature a medium pathlength a fast run time and excellent resolution. In fixed-angle rotors tubes are held at a constant angle during the spinning operation typically between 23 38 from vertical. Theyre commonly used at higher speeds for small to large volumes for pelleting applications such as bacteria and yeast to recover.