Fitc annexin v apoptosis detection kit ii

The TACS Annexin V-FITC Apoptosis Detection Kit is FITC is supplied with TACS Annexin V-FITC an optimized binding buffer and Propidium Iodide. Analysis is by flow cytometry or.

The Annexin V-FITC apoptosis detection kit allows the detection and discrimination between apoptotic and necrotic cells.

Fitc annexin v apoptosis detection kit ii. This annexin FITC Apoptosis detection kit is a highly sensitive and specific fluorometric assay kit for measuring Annexin V in apoptotic cells. Annexin V-FITC Apoptosis Detection Kit II MSDS material safety data sheet or SDS CoA and CoQ dossiers brochures and other available documents. Annexin V is a 35-36 kDa Ca2 dependent phospholipid-binding protein that has a high affinity for PS and binds to cells with exposed PS.

Annexin V may be conjugated to fluorochromes including FITC. This format retains its high affinity for PS and thus serves as a sensitive probe for flow cytometric analysis of cells that are undergoing apoptosis. This annexin FITC Apoptosis detection kit is a highly sensitive and specific fluorometric assay kit for measuring Annexin V in apoptotic cells.

Annexin V-FITC Apoptosis Detection Kit II MSDS material safety data sheet or SDS CoA and CoQ dossiers brochures and other available documents. FITC Apoptosis Detection Kit II Staining precedes the loss of membrane integrity which accompanies the latest stages of cell death resulting from either apoptotic or necrotic processes. The ApoDETECT Annexin V-FITC Kit provides all the reagents needed to detect the apoptotic cells.

Annexin V is a Ca 2 -dependent phospholipid binding protein. This protein can bind to a variety of phospholipids but it has the highest affinity for phosphatidylserine PS. During late-stage apoptosis loss of cell membrane integrity allows Annexin V binding to cytosolic PS as well as cell uptake of PI and 7-AAD.

Annexin V staining paired with 7-AAD or PI is widely used to identify apoptotic stages by flow cytometry. Annexin V a 35-36 kDa phospholipid-binding protein has a high affinity for PS KDa7nmoll. Once Annexin V is bound to the apoptotic cell it acts as a beacon for cells that have undergone apoptosis.

Annexin V is often conjugated to FITC so the amount of signal can be measured and analyzed from labeled cells using a flow cytometer. ApoScreen Annexin V Apoptosis Kit-FITC SB Cat. 10010-02 employs a fluorescein-conjugated Annexin V Annexin V-FITC in concert with propidium iodide PI to evaluate subpopulations of cells undergoing apoptosis.

During the early stages of apoptosis cells begin to display phosphatidylserine PS on the outer cell membrane where it is readily detectable by staining the cells with Annexin V-FITC. Product overview Annexin V-FITC Apoptosis Staining Detection Kit ab14085 is used in a 10 min one-step staining procedure to detect apoptosis by staining phosphatidylserine molecules which have translocated to the outside of the cell membrane. Analysis is by flow cytometry or.

Description BioLegends FITC Annexin V Apoptosis Detection Kit with PI has been specifically designed for the identification of apoptotic and necrotic cells. Annexin V or Annexin A5 is a member of the annexin family of intracellular proteins that binds to phosphatidylserine PS in a calcium-dependent manner. The TACS Annexin V-FITC Apoptosis Detection Kit is FITC is supplied with TACS Annexin V-FITC an optimized binding buffer and Propidium Iodide.

Cells are harvested and washed then incubated with an TACS Annexin V-FITC in binding buffer for 15 minutes at room temperature after which cells can be directly analyzed. Annexin V-FITC Apoptosis Detection Kit II SDS Safety Data Sheet for Annexin V-FITC Apoptosis Detection Kit II CBA059. Material Safety Data Sheet or SDS for Annexin V-FITC Apoptosis Detection Kit II CBA059 from MilliporeSigma for download or viewing in the browser.

The Annexin V-PE Apoptosis Detection Kit contains the bright orange-red PE fluorescent probe that can be easily detected by flow cytometry or fluorescence microscopy. Components K128-25 K128-100 K128-400 Part Number 25 assays 100 assays 400 assays Annexin V-PE 125 µl 500 µl 2. The Annexin V-Cy3 kit uses the dye Cy318 as the fluorochrome conjugated with annexin V.

By microscopy Cy318 fluoresces more brightly than the FITC conjugate. The kit includes the non-fluorescent compound 6-carboxyfluorescein diacetate 6-CFDA which enters the cell and is hydrolyzed by the esterases present in living cells to the. Features Annexin V-FITC Apoptosis Detection Kit contains ready-to-use solutions Annexin V-FITC conjugate propidium iodide PI.

The kit can identify apoptotic and necrotic cells Detect by flow cytometry or fluorescence microscopy No need to fix cells. The Annexin V-FITC apoptosis detection kit allows the detection and discrimination between apoptotic and necrotic cells. ANNEXIN V FITC Kit - Apoptosis Detection Kit PN IM3546 200 tests 12 IM3546 2009-01-30 For Research Use Only.

Not for Use in Diagnostic Procedures. SPECIFICITY The ANNEXIN V-FITC Kit is an apoptosis detection kit based on the binding properties of annexin V to phosphatidylserine PS and on the DNA-intercalating capabilities of. 活性染料可用于从早期凋亡细胞Annexin V 阳性活性染料阴性中分辨出这些晚期凋亡和坏死细胞Annexin V活性染料阳性 注意不建议将 Fixable Viability Dye eFluor 450 与 Annexin V Apoptosis Detection Kit 联用.

Annexins sind eine Familie Calcium-abhängiger Phospholipid-bindender Proteine die an Phophatidylserin binden PS. Die Externalisierung von Phosphatidylserin-Resten an die äußere Plasmamembran apoptotischer Zellen ermöglicht die Detektion mittels Annexin V. Wenn die apoptotischen Zellen an das markierte Annexin V gebunden sind können sie mit Fluoreszenz.