Muse cell cycle reagent

0500 3115 MuseCell Cycle Kit Catalog No. Not for use in diagnostic procedures.

G0G1 S and G2M of the cell cycle.

Muse cell cycle reagent. The Muse Cell Cycle Kit allows for easy and rapid quantitative measurements of the percentage of cells in the G0G1 S and G2M phases of the cell cycle. The Muse Cell Cycle Assay uses a premixed reagent which includes the nuclear DNA intercalating stain propid- ium iodide PI and RNAse A in a proprietary formulation. PI discriminates cells at different stages of the cell cycle.

Ethanol-fixed cells are treated with a premixed Muse Cell Cycle Reagent and acquired using the Muse Cell Cycle software module Figure 1. Muse Cell Cycle Assay Features Quick determination of cells in all 3 phases of cell cycle Premixed single reagentno reagent preparation needed Highly simplified acquisition and analysis. The Muse Cell Cycle Assay uses a premixed reagent which includes the nuclear DNA intercalating stain propidium iodide PI and RNAse A in a proprietary formulation.

PI discriminates cells at di fferent stages of the cell cycle based. Distribution of cells in different stages of cell cycle was analyzed by flow cytometry using the Muse Cell Cycle Kit from EMD Millipore Bioscience Darmstadt Germany. The kit utilizes propidium iodide PI staining to allow quantitative measurements of percentage of cells in the G0G1 S and G2M phases on the Muse Cell Analyzer EMD Millipore Bioscience.

The Muse Cell Dispersal Reagent 100 Tests Part Number. MCH100107 is an enzymatic reagent that has been formulated to gently disaggregate the cells that have aggregated in clumps in order to obtain uniform suspensions for cell counting. Healthy Jurkat cells were mixed with heat-killed Jurkat cells and stained with Muse Count Viability Reagent and then analyzed on the Muse Cell Analyzer.

Data output include summary data not shown and optional dot plots shown here. Reported statistics include viable cellsmL viability and the total cellsmL. G0G1 S and G2M of the cell cycle.

Muse Cell Dispersal Reagent Enzymatic reagent that gently disaggregates clumped cells in suspension improving the accuracy and precision of cell counts. The formulation of CDR has been optimized for use with Muse Count Viability Reagent. The Muse Cell Cycle Reagent contains dyes that may be carcinogenic andor mutagenic.

Exercise standard precautions when obtaining handling and disposing of potentially carcinogenic and mutagenic reagents. Refer to the MSDS for specific information on hazardous materials. Add 200 μL of Muse Cell Cycle Reagent to each tube.

We have used Muse cell cycle analysis kit to Muse Cell Analyzer. I have attached a file corresponding to the analysis of an untreated adherent cell line with Annexin V reagent at Muse Cell. Flowchart of Muse cell collection Polarizer.

Muse cell SSEA-3 positive 1. Preparation of cell suspension 2. Muse cell staining with anti-SSEA-3 antibody primary antibody Anti-SSEA-3 Rat IgM Staining of cell suspensions SSEA- 3 Adhesion culture Detachment Cell suspension Intracellular membrane Figure.

Staining image of SSEA-3 Anti -SSEA 3. Luminex MUSE Cell Analyzer MLSC 222 4 Users Guide to the CSULB Luminex MUSE Cell Analyzer Essential system specifications Lasers. 532nm Green laser Information on fluorophore excitation and emission frequencies is available on websites listed in the links section J of this guide.

Red or Yellow fluorescence. Muse Cell Cycle Kit MCH100106 To determine the cell cycle phases of cellular samples For Research Use Only. Not for use in diagnostic procedures.

Storage Conditions Store the Muse Cell Cycle Kit at 2 to 8C protected from light. Kit Components Muse Cell Cycle Reagent Part No. 4700-1495 100 testsbottle Materials Recommended Guava Muse Cell Analyzer.

MuseCell Analyzer Catalog No. 0500 3115 MuseCell Cycle Kit Catalog No. 1mL1106 cells のPBS でサスペンドする 5.

MCH100105 MuseTM Annexin V Dead Cell Kit MCH100106 MuseTM Cell Cycle Kit MCH100101 MuseTM Performance Check Kit MCH100104 MuseTM Count Viability Kit 200X for difficult cell MCH10010 MuseTM Cell Dispersant Reagent 4200-0140 MuseTM Instrument Cleaning Fluid ICF 0110-7455 MuseTM Flow Cell 0QP 8KCDNG GNN 8KCDNG GNN 0QV C GNN. Muse System은 일반적인 flow cytometry의 110 공간을 차지하는 microcapillary와 miniaturized optics을 사용하여 높은 성능의 cell 분석을 제공합니다. Muse Instrument는 단계별 조작이 매우 간편하며 누구나 쉽게 테스트를 실행할 수 있습니다.

최적화된 assay kit을 제공합니다. The Muse System delivers high-performance cell analysis using a microcapillary and miniaturized optics which occupy one-tenth the space of a typical flow cytometer. Laser-based fluorescence detection of each cell event can evaluate up to 3 cellular parameters.

Measure more accurately and reliably. View or download the Muse Cell Cycle Assay Kit MSDS Material Safety Data Sheet or SDS for MCH100106 from MilliporeSigma.