Pcdna3 1 myc his invitrogen

Annonse Products for genetic engineering amplification purification quantification analysis. 1 760 602 6500 E.

The figure below summarizes the features of the pcDNAŽ31myc-His- vectors.

Pcdna3 1 myc his invitrogen. Annonse Products for genetic engineering amplification purification quantification analysis. Solutions for every step in your journey to discovery. All pcDNA vectors contain a strong promoter for high-level expression in mammalian cells a choice of selection marker for generating stable cell lines and an epitope tag for easy detection with a monoclonal antibody and rapid purification on nickel-chelating resin.

Each vector is available in three reading frames to simplify cloning. 5 Sequencing 1 Primer. 5 Sequencing 1 Primer Sequence.

Invitrogen pcDNA31myc-His A B C Mammalian Expression Vectors Contain a strong promoter and a selection marker for generating stable cell lines Manufacturer. The control plasmid pcDNA31myc-HislacZ is the pcDNA31myc-His C vector with a 32-kb fragment containing the -galactosidase gene cloned in frame with the C-terminal peptide see page 9. It is included for use as a positive control for transfection expression purification and detection in the cell line of choice.

Control plasmid pcDNA31myc-His-lacZ is the pcDNA31myc-His- A vector with a 32 kb fragment containing the β-galactosidase gene cloned in frame with the C-terminal peptide see page 9. It is included for use as a positive control for transfection expression and detection in the cell line of choice. PcDNA31 myc-His- A B C 55 kb P C M V S V 4 0 o r i S V 4 0 p A pUC ori A m p i c i l l i n f1 or i BGHp A T7 N e o m y c i n Apa I Xba I myc epitope 6xHis Term.

Thr Arg Ala Ala Ala Thr Val Leu Asp Ile Cys Arg Ile Pro Pro His Trp Thr Ser Gly Ser Glu Leu Gly Thr Lys Leu Gly Pro Glu Gln Lys Leu Ile Ser Glu Glu Asp Leu Asn Ser Ala Val Asp His His His His His His TATA T7 promoterpriming site Apa I Xba I Xho I Not I BstX I EcoR V EcoR I BamH I Asp718 I BstX I Kpn I Hind III Apa I myc epitope. Invitrogen Corporation 1600 Faraday Avenue Carlsbad CA 92008 T. 1 760 603 7200 F.

1 760 602 6500 E. V790-20 and V795-20 Version K 10 November 2010 28-0104. Invitrogen pcDNA31 Mammalian Expression Vector Designed for high-level constitutive expression in a variety of mammalian cell lines Manufacturer.

This pcDNA31 vector is designed for high-level constitutive expression in a variety of mammalian cell lines. It contains a Geneticin selectable marker and a forward-orientation multiple cloning site. Three untagged versions of pcDNA31 available separately each with a different selectable marker Geneticin Zeocin or Hygromycin are.

Mammalian vector for expressing C-terminally Myc- and 6xHis-tagged proteins. For other reading frames use pcDNA31mycHis B or pcDNA31mycHis C. To see this sequence with restriction sites features and translations please.

The pcDNA6V5-His vectors are shipped on wet ice. Upon receipt store vectors at 20C. Store blasticidin powder at 20C.

Kit Contents The contents for each kit are listed below. Item Composition Amount V220-01 pcDNA6V5-His A B and C 40 μL of 05 gμL vector in 10 mM Tris-HCl 1 mM EDTA pH 80 20 g. PcDNA31-Myc-His RTN3A1 was a gift from Jimtong Horng Addgene plasmid 31087.

RRIDAddgene_31087 For your References section. Reticulon 3 binds the 2C protein of enterovirus 71 and is required for viral replication. The figure below summarizes the features of the pcDNAŽ31myc-His- vectors.

Details of the multiple cloning sites for pcDNAŽ31myc-His- A B and C are shown on pages 5-7. PcDNA31Myc-His A pcDNA31Myc-HisA. HindIII 1 911.

HinfI 16 173 660 869 988 1576 1598 2749 2883. 2935 2993 3030 3450 3515 3590 3986 4500. HpaII 22 1424 2076 2169 2246 2268 2296 2427 2517.

2584 2765 3040 3048 3333 3822 3969 3995. 4185 4586 4620 4687 4797 5039. Full sequence for pcDNA31myc-His B shared on Benchling.

Annonse Products for genetic engineering amplification purification quantification analysis. Solutions for every step in your journey to discovery.