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19 LightCycler FastStart DNA Master SYBR Green I General Considerations Two-Step RT-PCR LightCycler FastStart DNA Master SYBR Green I can also be used to perform two-step RT-PCR. 03 515 869 001 1 kit 96 reactions of 20 μl final volume each.
January 2017 Easy-to-use hot start reaction mix for PCR using the LightCycler Carousel-Based System Cat.
Roche lightcycler sybr green master mix. The LightCycler 480 SYBR Green I Master is a one-component hot start reaction mix for PCR. It contains FastStart Taq DNA Polymerase and DNA double-strand-specific SYBR Green I dye for product detection and characterization. LightCycler FastStart DNA Master SYBR Green I Easy-to-use hot start reaction mix for PCR using the LightCycler Carousel-Based System.
For life science research only. Not for use in diagnostic procedures. Documents The LightCycler RNA Master SYBR Green I provides reagents including RNA Master Mix with buffer SYBR Green I nucleotides and enzyme a Mn OAc 2 stock solution and PCR-grade Water.
The kit is specially designed for use with the LightCycler 20 System Instruments. LightCycler FastStart DNA Master PLUS SYBR Green I Easy-to-use hot start reaction mix for PCR using the LightCycler Carousel-Based System. The LightCycler EvoScript RNA SYBR Green I Master is designed for sensitive high-specificity high-precision one-step RT-qPCR especially for gene expression assays.
Ease-of-use convenience speed reduced error Result consistency stability and tolerance of targets of varying secondary structure. How to Use this Product y Version. 13 Two-step RT-PCR LightCycler 480 SYBR Green I Master can also be used to perform two-step RT-PCR.
In two-step RT-PCR the reverse transcription of RNA into cDNA is separated from the other reaction steps and is performed outside the. Roche Applied Science 4 Application LightCycler 480 SYBR Green I Master is designed for research studies. When used with the LightCycler 480 System this kit is ideally suited for hot-start PCR applications.
In combination with the LightCycler 480 System and suit- able PCR primers this kit allows very sensitive detection and quantification of. How to Use this Product y Version. 19 LightCycler FastStart DNA Master SYBR Green I General Considerations Two-Step RT-PCR LightCycler FastStart DNA Master SYBR Green I can also be used to perform two-step RT-PCR.
In two-step RT-PCR the reverse transcription of RNA into cDNA is separated from the other reaction steps and is performed outside the. Water 10 Primer - Forward 5 µM Stock 05 µM 20 Primer - Reverse 5 µM Stock 05 µM 20 LightCycler 480 SYBR Green I Master 1x 100 Total150 Add. Roche LightCycler Nano.
Master Mix SYBR Green PCR Master Mix SYBR GreenER qPCR SuperMix Universal PowerUp SYBR Green Master Mix PowerTrack SYBR Green Master Mix 4472903 4472908 4472913 4472918 4472919 4472920 4385610 4385612 4385616. The FastStart Essential DNA Green Master is a one-component hot start reaction mix for qualitative and quantitative PCR. It contains FastStart Taq DNA Polymerase and double-stranded DNA specific SYBR Green I dye for product detection and characterization.
Applied Biosystems SYBR Green PCR Master Mix combines SYBR Green I dye AmpliTaq Gold DNA Polymerase dNTPs with dUTP Passive Reference 1 and optimized buffer in the convenience of a single vial. Premixed components stored at 28C significantly reduce assay setup time. Fast SYBR Green Master Mix.
Executed on Roche LightCycler 480 Real-Time PCR System For safety and biohazard guidelines refer to the Safety section in the Fast SYBR Green Master Mix Protocol PN 4385372. Read the MSDS and follow the handling instructions. FastStart Universal SYBR Green Master Rox y Version.
August 2016 2x concentrated ready-to-use hot start master mix for qPCR and qRT-PCR using the SYBR Green I detection format on real-time PCR instruments except the LightCycler Instruments Cat. 04 913 850 001 4 x 125 ml 200 reactions of 50 μl final volume each. Roche lightcycler 480 sybr green i master mix kit Lightcycler 480 Sybr Green I Master Mix Kit supplied by Roche used in various techniques.
86100 based on 1 PubMed citations. ZERO BIAS - scores article reviews protocol conditions and more. Not for use in diagnostic procedures.
R LightCycler FastStart DNA Master PLUS SYBR Green I y Version. January 2017 Easy-to-use hot start reaction mix for PCR using the LightCycler Carousel-Based System Cat. 03 515 869 001 1 kit 96 reactions of 20 μl final volume each.
The SYBR Green I dye contained in KAPA SYBR FAST qPCR Master Mix 2X and ROXfluorescein dyes depending on kit configuration are light sensitive. Exposure to direct light for an extended period of time will result in loss of fluorescent signal intensity. KAPA SYBR FAST qPCR Master Mix 2X is stable through 30 freeze-thaw cycles.
PowerTrack SYBR Green Master Mix is a pre-formulated optimized universal 2X master mix for real-time PCR workflows with built-in visual dyebased indicators for accurate reaction set up. Coupled with user-supplied primer sets and template PowerTrack SYBR Green Master Mix is designed to amplifiy targets for accurate gene expression analysis. The 2x mix contains SYBR GreenER dye AmpliTaq DNA Polymerase UP dNTPs with dUTPdTTP blend heat-labile UDG ROX passive reference dye and optimized buffer components.
Contains 1 X 1 mL tube sufficient for 100 20 µL reactions 10 µL.