Spe1 restriction site sequence

Sequence Help SPE1 YKL184W Sequence Sequences and genome annotation information for Reference Strain S288C and a select set of Alternative References. Restriction endonucleases in DNA sequences.

Activity at 37C for Restriction Enzymes with Alternate Incubation Temperatures.

Spe1 restriction site sequence. Activity at 37C for Restriction Enzymes with Alternate Incubation Temperatures. Activity of Restriction Enzymes in PCR Buffers. Cleavage Close to the End of DNA Fragments.

Digestion of Agarose-Embedded DNA. Info for Specific Enzymes. NEBuffer ActivityPerformance Chart with Restriction.

Cerevisiae Reference Genome sequence is derived from laboratory strain S288C. Download DNA or protein sequence view genomic context and coordinates. Click Sequence Details to view all sequence information for this locus including that for other strains.

Sequence Help SPE1 YKL184W Sequence Sequences and genome annotation information for Reference Strain S288C and a select set of Alternative References. SpeI is a restriction endonuclease used for molecular biology applications to cut DNA at the recognition site 5-ACTAGT-3 generating DNA fragments with 5-cohesive ends. Other Notes Supplied with 10x Restriction Enzyme Buffer SH B3657.

SpeI has a High Fidelity version SpeI-HF NEB R3133. High Fidelity HF Restriction Enzymes have 100 activity in rCutSmart Buffer. Single-buffer simplicity means more straightforward and streamlined sample processing.

Thermo Scientific BcuI SpeI restriction enzyme recognizes ACTAGT sites and cuts best at 37C in Tango buffer isoschizomers. See Reaction Conditions for Restriction Enzymes for a table of enzyme activity conditions for double digestion and heat inactivation for this and other restriction. Sequence of DNA recognized by the enzyme and to which it specifically binds.

Cutting site and DNA products of the cut. The recognition sequence and the cutting site usually match but sometimes the cutting site can be dozens of nucleotides away from the recognition site. Recognition sequence reaction conditions heat denaturation and microbial source for SpeI restriction enzyme.

Promoter T7 Tag Fusion Protein. Histidine C terminal on backbone Cloning Information Cloning method Restriction Enzyme 5 cloning site NcoI not destroyed 3 cloning site XhoI not destroyed. Welcome to RestrictionMapper - on line restriction mapping the easy way.

Maps sites for restriction enzymes aka. Restriction endonucleases in DNA sequences. Also does virtual digestion.

The sequence in green is a Pst1 restriction site and that in orange is the Spe1 restriction site. The region in purple is the sequence of shared overlap with the forward primer. As can be seen there have been randomised nucleotides introduced so as to create a library of varying promoters.

Plasmid pMJ915v2Nterm Spe1 Cterm Age1 site from Dr. Jennifer Doudnas lab is published in Nat Biotechnol. This plasmid is available through Addgene.

The recognition of the substrate sequence by a restriction endonuclease depends on the direct contacts between each nucleotide of the recognition sequence and the relevant amino acid residues of endonucleases. The sulfur replacement affects the electron density on the phosphate group leading to the changes in the cleavage activity. Within the sequence GATC.

5-methylcytosine and 5-hydroxymethylcytosine are also inhibiting. Recognition Sites TCTAGA indicates methylation sensitivity. Specificity Star activity The Xba I sequence specificity is relaxed at low ionic strength or by addition of glycerol ethanol or DMSO to the incubation mixture.

New restriction sites can be generated by ligation of DNA fragments with compatible cohesive or blunt ends. These new restriction sites may be generated by. Cleavage followed by fill-in of 5 overhangs to generate blunt ends.

Cleavage with two restriction endonucleases that produce blunt ends. Cleavage with two restriction endonucleases that. Restriction site fragment data can be coded as characters and character states in a phylogenetic analysis.

For example given that the restriction site maps of Figure 1410 are correct the presence or absence of these sites can be coded as characters as seen in Figure 1411Restriction site analysis contains far less data than complete DNA sequencing accounting only for the presence or. DNA Restriction Enzymes from Takara such as SpeI are high-quality. Perform restriction enzyme digestion with reliable restriction endonucleases.

1086B contains 5 of Cat. Please refer to Cat. 1086A for complete product documentation and resources.

Our products are to be used for Research Use Only. Thermo Scientific SdaI SbfI restriction enzyme recognizes CCTGCAGG sites and cuts best at 37C in its own unique buffer. See Reaction Conditions for Restriction Enzymes for a table of enzyme activity conditions for double digestion and heat inactivation for this and other restriction.

All HF-restriction enzymes come with Gel Loading Dye Purple 6X. Enjoy the enhanced performance and added value of our engineered enzymes at the same price as the native enzyme. Engineered for improved performance.

100 activity in rCutSmart Buffer.