Syto 9 excitation emission

SYTO 9 stain S-34854 has been shown to stain live and dead Gram-positive and Gram-negative bacteria and it is a component of the. SYTO green-fluorescent dye bound to nucleic acid is shown in the Appendix Figures 14 pages 57.

3 Fluorescence Microplate Readers Reagents Kit Contents for Viability Kit L7012.

Syto 9 excitation emission. SYTO 9 stain has a high affinity for DNA and exhibits enhanced fluorescence upon binding with an excitation maximum at 483 nm and fluorescence emission maximum at 503 nm. SYTO 9 stain is particularly useful as a nuclear counterstain for bacterial assays since it stains both live and dead Gram-positive and Gram-negative bacteria. The easiest way to differentiate a bacterial population from other small particles and electronic noise is to label the bacteria with a fluorescent stain and use the fluorescence emission to identify the population of interest.

SYTO 9 is a great stain to label all cells and is detected in the FITC channel. I have a LIVEDEAD BacLight Bacterial. Excitation and Emission Spectra To establish emissionexcitation spectra SYTO9 and PI were added to solutions of DNA.

The manufacturer rec-ommends that PI should be used at a concentration six times that of SYTO9 in initial trials with the kit. In accor-dance with this recommendation the excitationemis-. Excitation filter Dichroic mirror Emission filter Remarks.

JNO-BB 48030 465495 505 LP 53540 515555 Medium Excitation band Bandpass Emission. 47040 450490 495 LP 52550 500550 Wide Excitation band Bandpass Emission. 48040 460500 510 LP 53550 510560 Wide Excitation band Bandpass Emission.

Propidium IodideSyto 9 FLUOROPHORES GUIDE L I V E D E A D B A C L I G H T B A C T E R I A L V I A B I L I T Y K I T S Syto 9 Excitation Syto 9 Emission Optical Kit Excitation Optical Kit Emission 400 500 600 700 Propidium Iodide Excitation Propidium Iodide Emission Optical Kit Excitation Optical Kit Emission 400 500 600. For SYTO9 emission spectra were collected with excitation at 420 nm and the absorption spectrum was measured by emissions at 580 nm. For PI the emission spectrum was collected with excitation at 488 nm and the absorption spectrum was collected with emissions at 640 nm.

SYTO green-fluorescent dye bound to nucleic acid is shown in the Appendix Figures 14 pages 57. Absorption nm Emission nm QY DNA QY DNA RNA DNA RNA RNA SYTO 9 S34854 485 486 498 501 058 ND SYTO 11 S7573 508 510 527 530 049 039 SYTO 12 S7574 499 500 522 519 009 013 SYTO 13 S7575 488 491 509 514 0. Thus with an appropriate mixture of the SYTO 9 and propidium iodide stains bacteria with intact cell membranes ie live stain fluorescent green whereas bacteria with damaged membranes ie dead stain fluorescent red.

The excitationemission maxima for these dyes are approximately 480500 nm for SYTO 9 stain and 490635 nm for propidium. With SYTO dyes generally show cytoplasmic or mi to chon dri al staining as well as nuclear staining. The SYTO green-fluorescent nucleic acid stains have prov en valuable in a broad range of research applications.

SYTO 9 stain S-34854 has been shown to stain live and dead Gram-positive and Gram-negative bacteria and it is a component of the. OsII complexes the wavelength of excitation was 475 nm and the emission was measured at 745 nm. For Syto 9 the wavelength of excitation was 475 nm and the emission was measured at 500 nm.

Emission decay parameters for OsII complexes 50 μM or Syto 9 2 μM measured by time-resolved emission in calf thymus DNA. Excitation max and Emission max of the fluorescent molecules are subjected to. Propidium iodide or PI is a fluorescent intercalating agent that can be used to stain cells and nucleic acidsPI binds to DNA by intercalating between the bases with little or no sequence preference.

When in an aqueous solution PI has a fluorescent excitation maximum of 493 nm blue-green and an emission maximum of 636 nm red. SYTO 9 green fluorescent nucleic acid stain has been shown to stain live and dead Gram-positive and Gram-negative bacteria and it is a component of the LIVEDEAD Bac Light Bacterial Viability Kits L-7007 L-7012 L-13152. Analysis of Bacteria Bacterial Viability Cytotoxicity Analysis Cell Analysis Cell Tracing Tracking Cell.

The excitationemission maxima for these dyes are about 480500nm for SYTO 9 stain and 490635nm for propidium iodide. Equipment 2 Fluorescence Spectroscopy. 3 Fluorescence Microplate Readers Reagents Kit Contents for Viability Kit L7012.

SYTO 9 dye 334 mM Component A 300 μL solution in. Excitation and Emission Peaks Dye Excitation Max nm Emission Max nm 6-Carboxyrhodamine 6G 525 555 7-Aminoactinomycin D 7-AAD 546 647 7-Hydroxy-4-methylcoumarin 360 455 Acid Fuchsin 540 630 Acridine orange DNA 500 526 Acridine orange RNA 460 650 Acridine Red 455-600 560-680 Acridine Yellow 470 550. SYTO 9DNA Excitation Spectra.

SYTO 9DNA Emission Spectra. SYTO9 The Mix contains fluorescent DNA dye SYTO9 which after binding to doublestranded dsDNA becomes strongly fluorescent with maximal excitation at 485 nm and emission at 498 nm green light. Invitrogens patented Syto dyes are cell-permeant cyanine dyes that bind to nucleic acids.

Several Syto dyes are available with varying cell permeability fluorescence enhancement upon binding to nucleic acids excitation and emission spectra and nucleic acid selectivity and binding affinity. The Syto 60 stain has absorption and fluorescence emission maxima of 652678 nm.