Zic hilic stationary phase

Of polar analytes on HILIC stationary phase depends on the intesity of polar interactions between polar groups of analytes and polar moieties from stationary phase 34. Separation selectivity is favored by the 11 zwitterion charge balance which makes the ZIC -HILIC Column overall neutral with weak but important ionic interactions.

Separation selectivity is favored by the 11 zwitterion charge balance which makes the ZIC-HILIC column overall neutral with weak but important ionic interactions.

Zic hilic stationary phase. ZIC-HILIC 100 x 21 mm 35 μm100 Å 1504440001 Mobile Phase. 20 vv 100 mM NH 4 Ac buffer pH 45. MS-ESI- with SIM at 1172 mz Drying-gas flow rate Drying-gas temperature Capillary voltage.

10 Lmin 300C 30 kV. Refractive index cell 9 μL 40C. The zwitterionic ZIC-HILIC column provided better chromatographic performance over the silica stationary phase with 14 standards achieving good quality peaks compared to the 7 with the silica column.

Only 2 standards were undetected with the ZIC-HILIC column compared to the 14 undetected with the silica column. The ZIC-HILIC column contains a zwitterionic sulfoalkylbetaine stationary phase covalently attached to porous silica. The amide column is based on the ethylene bridged hybrid BEH particle technology with trifunctionally-bonded amide phase.

ZIC is a registered trademark of Merck KGaA The functional group of the ZIC-HILIC and ZICpHILIC stationary phases. The retention processes in HILIC illustrated by hydrophilic partitioning and. The silica-based ZIC -HILIC sorbent has a bonded stationary phase consisting of a highly polar permanent zwitterion.

Separation selectivity is favored by the 11 zwitterion charge balance which makes the ZIC -HILIC Column overall neutral with weak but important ionic interactions. In principle HILIC stationary phases can be divided into three different groups neutral charged and zwitterionic. Different classes of stationary phases for HILIC.

Neutral top charged middle and zwitterionic bottom. Of polar analytes on HILIC stationary phase depends on the intesity of polar interactions between polar groups of analytes and polar moieties from stationary phase 34. At their turn these interactions depend on the composition of the mobile phase and temperature 59.

Zwitterionc liquid chromatography ZIC-HILIC is a particular HILIC. Zwitterionic HILIC stationary phases are particularly versatile and they can be considered the HILIC all purpose phases. Some of the zwitterionic phases are commercialized under the trademarks ZIC-HILIC ZIC-cHILIC KS-polyMPC Obelisc R and Obelisc N.

ZIC-HILIC Analytical PEEK Columns 35µm and 5µm 200Å Column id. Mm Column Length mm Guard Column 50 100 150 250 PEEK 35µm Phase 539 704 747 - 441 21 1504450001 1504470001 1504480001 - 15043400011 5pk 589 - 747 - 559 46 1504460001 - 1504490001 - 15043600012 3pk 5µm Phase 490 642 678 823 441. Schematic illustation of the zwitterionic ZIC-HILIC betain sulfonate stationary phase.

Schematic illustation of the inverted zwitterionic ZIC-cHILIC cholinate stationary phase. A chemical extention of both the hydroxyethyl amide and ZIC chemistries iHILIC FUSION FUSION offer features of both of the above approaches. Syncronis HILIC stationary phase carries a zwitterionic functional group of the sulfobetaine type monomerically bonded to highly pure high surface area silica.

The sulfobetaine zwitterion has both positive quaternary ammonium and negative sulfonic acid groups in a. The HILIC stationary phases SPs like Normal Phase Liquid Chromatography NPLC contain bare silica or modified silica gels with different polar functional groups Some designed various SPs such as diol TSK gel Amide-80 amino ZIC HILIC Click maltose and other polar ligands especially for HILIC. The silica-based ZIC-HILIC sorbent has a bonded stationary phase consisting of a highly polar permanent zwitterion.

Separation selectivity is favored by the 11 zwitterion charge balance which makes the ZIC-HILIC column overall neutral with weak but important ionic interactions. Peptide retention time prediction models have been developed for zwitter-ionic ZIC-HILIC and ZIC-cHILIC stationary phases pH 45 eluents using proteomics-derived retention datasets of. Application of HILIC stationary phase to determination of dimethindene maleate in topical gel.

Matysová L1 Havlíková L Hájková R Krivda A Solich P. 1Department of Analytical Chemistry Charles University Hradec Králové Czech Republic. Ted ZIC -HILIC stationary phase.

The selectivity of the ZIC-HILIC column can be tuned by varying the amount of organic modifier the pH and the low ionic strength of the eluent where an increase in the organic solvent content or a decrease in the ionic strength promotes retention. Separation of methacryl-amide and acrylamide on a ZIC-HILIC column 250 x 46 mm 5 um 200 Å PN.