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VECTASHIELDHardSet Mounting Medium prevents rapid photobleaching of fluorescent proteins and dyes. 222 n-Propyl gallate NPG.
Mounted slides should be stored at 4 degrees C protected from light.
Antifade mounting medium invitrogen. ProLong Gold antifade mountant has become a widely used antifade reagent for our Invitrogen high-performance fluorescent dyes and stains because it preserves signal across the entire visible spectrum and causes little or no quenching of the initial signal. Mounting Media and Antifades Preserve the fluorescent signal from your sample Loss of fluorescence through irreversible photobleaching processes can lead to a significant reduction in sensitivity particularly when target molecules are of low abundance or when excitation light is of high intensity or long duration. ProLong Gold Antifade Mountant is a liquid mountant applied directly to fluorescently labeled cell or tissue samples on microscope slides.
It contains chemical components designed to protect fluorescent dyes from fading photobleaching during fluorescence microscopy experiments and delivers this protection without significantly quenching the initial fluorescence signal. ProLong Gold Antifade Mountant is a liquid mountant applied directly to fluorescently labeled cell or tissue samples on microscope slides. It is formulated without DNA stain or with the blue DNA stain DAPI or with the deep red DNA stain SYTOX Deep Red.
ProLong Gold mountant contains chemical components designed to protect fluorescent dyes from fading photobleaching during fluorescence. ProLong Gold Antifade Mountant is a curing mountant that forms a near-perfect optical path 147 RI and allows longer-term storage of the sample. Ready-to-use room temperatures storage and no mixing required-just apply a drop to the sample add a coverslip cure and image.
ProLong Gold mountant is available with or without DAPI nuclear stain. Invitrogen ProLong Glass Antifade Mountant ProLong Glass Hard-Set Antifade Mountant is a glycerol-based hard-setting ready-to-use mountant that can be applied directly to fluorescently labeled cells or tissue samples on microscope slides. 7100 - 24200 Products 3.
VECTASHIELDHardSet Mounting Medium prevents rapid photobleaching of fluorescent proteins and dyes. After approximately 15 minutes at room temperature the mounting medium will harden the coverslip will become immobilized and optimal antifade ability and refractive index of 146 will be achieved. Mounted slides should be stored at 4 degrees C protected from light.
For mountants with 152 refractive index optimized for oil immersion objective imaging try curing mountant Prolong Glass Antifade Mountant or non-curing SlowFade Glass Soft-set Antifade Mountant. ProLong Diamond Antifade Mountant comes already mixed and ready to use in a dropper bottle or 10-mL bottle with or without DNA stain. Invitrogen Fluoromount-G Mounting Medium Designed for use in mounting slides following immunofluorescent staining.
Water-soluble Fluoromount-G is used to mount slides in which the final step of staining is aqueous. It forms a semi-permanent seal for prolonged storage of slides at 2 to 8C. Phenylenediamine as an anti-fading reagent in mounting media may result in weak and diffused fluorescence after storage of the stained slides.
222 n-Propyl gallate NPG. Anti-fade mounting medium with DAPI for visualizing the nuclei Invitrogen. HCMECD3 cells are passaged with trypsin-EDTA 025.
Invitrogen Carlsbad CA typically twice a week from one dish to four dishes of equal size or from one P100 dish to four 6-well plates. In order to pass the cells a. Homemade Antifade Solution Mounting media is cheap and easy to make yourself and gives better results than many of the expensive commercial options out there.
You only need three things from a fluorescence mounting mediait should buffer your sample and fluorophores prevent photobleaching and have a high refractive index. Finally sections were washed in PBS mounted on Fisher Superfrost polarized slides and coverslipped with Prolong Antifade mounting medium Invitrogen. Sections were photographed and processed with a Zeiss Axio Imager Z1 Microscope equipped with a.
ProLong Gold Antifade Mountant is a liquid mountant applied directly to fluorescently labeled cell or tissue samples on microscope slides. It contains chemical components designed to protect fluorescent dyes from fading photobleaching during fluorescence microscopy experiments and delivers this protection without significantly quenching the initial fluorescence signal. Novus Biologicals VECTASHIELD R Antifade Mounting Medium with DAPI is a unique stable formula for preserving fluorescence.
Novus Biologicals H1200NB Catalog No. Slides were mounted in antifade mounting medium 90 glycerol 01 m Tr is pH 90 02 m n-propyl gallate. The primary antibody step was omitted for some sections as a negative control.
Immunostained sections were examined under epifluorescent illumination and digital images of each section were obtained. Fibres were classified as. ProLong Gold Antifade Mountant is a curing mountant that forms a near-perfect optical path 147 RI and allows longer-term storage of the sample.
Ready-to-use room temperatures storage and no mixing required-just apply a drop to the sample add a coverslip cure and image. ProLong Gold mountant is available with or without DAPI nuclear stain. Sections were washed with PBS and mounted on Fisher Superfrost polarized slides and coverslipped with Prolong Antifade mounting medium Invitrogen.
Sections were visualized and photographed with a Zeiss Axio Imager Z1 Microscope equipped with a digital camera and images were processed with Zeiss AxioVision software. The samples were covered with Prolong gold mounting medium containing DAPI Invitrogen catalog no. P36935 and were allowed to dry overnight in the dark at room temperature.
Images were captured using a Laser Scanning Microscope LSM 510 Meta Carl Zeiss Jena Germany and analyzed with Zeiss LSM Image Examiner Version 40091.